Atypical Mitogen-activated Protein Kinase Phosphatase Implicated in Regulating Transition from Pre-S-Phase Asexual Intraerythrocytic Development of Plasmodium falciparum

dc.contributor.authorCampbell, Christopher
dc.date.accessioned2020-10-15T14:42:53Z
dc.date.available2020-10-15T14:42:53Z
dc.date.issued2013
dc.description.abstractIntraerythrocytic development of the human malaria parasite Plasmodium falciparum appears as a continuous flow through growth and proliferation. To develop a greater understanding of the critical regulatory events, we utilized piggyBac insertional mutagenesis to randomly disrupt genes. Screening a collection of piggyBac mutants for slow growth, we isolated the attenuated parasite C9, which carried a single insertion disrupting the open reading frame (ORF) of PF3D7_1305500. This gene encodes a protein structurally similar to a mitogen-activated protein kinase (MAPK) phosphatase, except for two notable characteristics that alter the signature motif of the dual-specificity phosphatase domain, suggesting that it may be a low-activity phosphatase or pseudophosphatase. C9 parasites demonstrated a significantly lower growth rate with delayed entry into the S/M phase of the cell cycle, which follows the stage of maximum PF3D7_1305500 expression in intact parasites. Genetic complementation with the full-length PF3D7_1305500 rescued the wild-type phenotype of C9, validating the importance of the putative protein phosphatase PF3D7_1305500 as a regulator of pre-S-phase cell cycle progression in P. falciparum.en_US
dc.identifier.citationBalu, B., Campbell, C., Sedillo, J., Maher, S., Singh, N., Thomas, P., Zhang, M., Pance, A., Otto, T. D., Rayner, J. C., & Adams, J. H. (2013). Atypical mitogen-activated protein kinase phosphatase implicated in regulating transition from pre-S-Phase asexual intraerythrocytic development of Plasmodium falciparum. Eukaryotic Cell, 12(9), 1171–1178. https://doi.org/10.1128/EC.00028-13en_US
dc.identifier.other
dc.identifier.urihttp://hdl.handle.net/20.500.12521/97
dc.identifier.urihttps://doi.org/10.1128/EC.00028-13
dc.language.isoenen_US
dc.publisherEukaryotic Cellen_US
dc.relation.ispartofseries12(9);1171-1178
dc.titleAtypical Mitogen-activated Protein Kinase Phosphatase Implicated in Regulating Transition from Pre-S-Phase Asexual Intraerythrocytic Development of Plasmodium falciparumen_US
dc.typeArticleen_US

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